Protein electrophoresis band issues refer to problems or irregularities that may arise during the process of separating proteins based on their electrical charge using electrophoresis. These issues may include the appearance of unexpected or abnormal bands, poor resolution, smearing, or distortion of the protein bands, among others. We summary several common issues for our customers’ reference to find out the reasons and to obtain accurate results and improve the quality of protein separation.
Smile band– band pattern curves upward at both sides of the gel
Cause
1. Center of the gel running hotter than either end
2. Power conditions excessive
Solution
① Buffer not mixed well or buffer in upper chamber too concentrated. Remake buffer, ensuring thorough mixing, especially when diluting 5x or 10x stock
② Decrease the power setting from 200 V to 150 V or fill lower chamber to within 1 cm of top of short plate
Vertical streaking of protein
Cause
1. Sample overloaded
2. Sample precipitation Solution
Solution
① Dilute sample, selectively remove predominant protein in sample, or reduce the voltage about 25% to minimize streaking
② Centrifuge sample before addition of SDS sample buffer, or decrease %T of the gel
③ The ratio of SDS to protein should be enough to coat each protein molecule with SDS, generally 1.4:1. It may require more SDS for some membrane protein samples
Band horizontally spreading
Cause
1. Diffusion of the wells prior to turning on the current
2. Ionic strength of the sample lower than that of the gel
Solution
① Minimize the time between sample application and turning on the power startup
② Use same buffer in sample as in the gel or the stacking gel
Protein bands distorted or skewed
Cause
1. Poor polymerization around wells
2. Salts in sample
3. Uneven gel interface
Solution
① Degas stacking gel solution completely prior to casting; increase ammonium persulfate and TEMED concentrations by 25%, for stacking gel or low %T, leave APS the same and double the TEMED concentration.
② Remove the salts by dialysis, desalting;
③ Decrease the polymerization rate. Overlay gels very carefully.
Beijing Liuyi Biotechnology Company Ltd manufactures a range of electrophoresis products that can help to solve the issues we maybe meet in our electrophoresis experiment.
Beijing Liuyi Biotechnology Company Ltd established in 1970, formerly known as Beijing Liuyi Instrument Factory, is a manufacturer of laboratory equipment and scientific instruments based in Beijing, China. With over 50 years experience in designing and manufacturing products for electrophoresis and life science research, it becomes a leading provider of laboratory instruments and solutions in China.The company’s products include a wide range of laboratory instruments, including horizontal nucleic acid electrophoresis tank, vertical protein electrophoresis tank/unit, black-box type UV analyzer, Gel Document Tracking Imaging Analyzer, and electrophoresis power supply. These products are widely used in research institutions, universities, and various industries, such as pharmaceuticals, biotechnology, and environmental monitoring.The company is ISO9001 & ISO13485 certified company and has CE certificates.
There are various kinds of vertical electrophoresis tanks for protein electrophoresis for analysis and identification of protein samples by polyacrylamide gel electrophoresis, and also for measuring samples’ molecular weight, purifying samples and preparing samples. These products are all welcomed in domestic and overseas market.
The electrophoresis power supply is an essential component of electrophoresis system, providing a steady and precise source of electrical current to drive the separation process. It typically delivers a constant voltage or constant current to the electrophoresis system, depending on the specific experimental protocol. It also allows the user to adjust the voltage or current output, as well as other parameters such as time and temperature, to optimize the separation conditions for a particular experiment.
For observing the gel, you can choose a UV Transilluminator WD-9403 series manufactured by Beijing Liuyi Biotechnology. A UV transilluminator is a laboratory instrument used to visualize and analyze DNA, RNA, and protein samples. It works by illuminating the samples with UV light, which causes the samples to fluoresce and become visible. There are several models of UV transilluminator offered for you by us. WD-9403A is specially for observing protein electrophoresis, and the WD-9403F is used for observing DNA and protein electrophoresis.
These series of products can serve from casting gel to observing gel according to your experiment requirements.Let us know your requirements, both OEM, ODM and distributors are welcomed. We will try our best to offer you our products and services.
If you have any purchasing plan for our products, please don’t hesitate to contact us. You can send us message at email [email protected] or [email protected], or please call us at +86 15810650221 or add Whatsapp +86 15810650221, or Wechat: 15810650221.
Post time: Mar-29-2023